Introduction

The Cell Immortalization Unit of the Josep Carreras Leukaemia Research Institute offers Infection of B-cells with Epstein–Barr virus (EBV) leads to more and subsequent immortalization. This is considered as the method of choice for generating lymphoblastoid cell lines (LCLs). Cell culture is an essential tool to study the fundamentals of genetic background variables. With the development of personalized medicine, this applies increasingly to the development and safety testing of drugs. Infection of B-cells with Epstein–Barr virus (EBV) leads to more and subsequent immortalization. This is considered as the method of choice for generating lymphoblastoid cell lines (LCLs). After successfully production of LCLs, different parameters including temperature, serum concentration, type of culture medium, and CO2 concentration must be evaluated on EBV-transformed B-cells. Our unit can produce LCLs and optimize condition.

Services

• This immortalization technology enables rapid, efficient, and reliable production of unlimited numbers of personalized cells.
• To produce control material for rare genetic disorders.
• Lymphocyte Immortalization technique let to preserve of DNA, RNA, and proteins samples, that appears to be a valid strategy for further studies.
• To determine optimized condition for reliable and reproducible LCLs from different sources.
• Testing drugs analysis.
• Allows us to have enough biological sample without having to access the patient again