Proteomics

Introduction

The Josep Carreras Leukaemia Research (IJC) Institute Proteomics Core is located on the Can Ruti campus, providing an exceptional translational research niche. The IJC Proteomics Core provides state-of-the-art mass spectrometry-based proteomics services, identifying, quantifying, and characterizing proteomes, proteins, and their post-translational modifications. We offer proteomics services to Institute scientists as well as outside public and private organizations, run by a highly committed team.

Services

The Proteomics Cores Resource provides a wide range of mass spectrometry services:

Differential Proteomics expression using label-free or label-based techniques (e.g., TMT)
Identification and localization of Post-Translational Modifications (e.g., phosphorylation, ubiquitinylation, acetylation, and methylation)
Protein-protein interaction (i.e., Pull-down, BioID, Crosslink)
Extracellular vesicles from liquid samples (plasma and saliva) and cells
Targeted protein-peptide identification and quantification
Top Down (intact protein)
Monodimensional SDS-PAGE
Protein and AND/RNA extraction by FastPrep-24 5G bead beater
Process automation-standardization by Robot Biomeik5 (Proteomics, Genomics, and basic research)
Data analysis (differential expression and system biology) and Scientific support

We encourage you to consult with us before starting your proteomics experiment. We will assist you in designing the best experiment to meet your objectives.

Equipment & Resources

The Proteomics Core is equipped with an Exploris 480 mass spectrometer (Thermo Scientific) and two chromatographs, Evosep One (Evosep) and Vanquish Neo (Thermo Scientific), offering a versatile pipeline depending on sample analysis requirements, and a robust computational infrastructure and software. Additionally, the core offers a process automation-standardization service by means of Robot Biomeik5 (Beckman) and FastPrep-24 5G bead beating grinder and lysis system (MP Biomedical).