Introduction

The Genomics Unit focuses on the application of high throughput genomic and epigenomic technologies to support cutting-edge biomedical research.

The facility houses specialized equipment to provide genomic services to the IJC community as well as to external customers. 

We have long standing experience in genome-wide characterization of DNA methylation profiles using Illumina Infinium Technology (Human MethylationEPIC v2.0 and Mouse Methylation BeadChips).

We offer different levels of service depending upon user needs, including experimental design, sample quality control, processing, and basic data analysis.

Services

Array-based applications

• DNA Methylation analysis
• SNP Genotyping and CNV analysis 

Equipment: Illumina iScan with Autoloader system & TECAN Freedom EVO liquid-handling robot

Next Generation Sequencing (NGS) applications

• Targeted resequencing
• Small genome sequencing
• Metagenomics (16S rRNA)

Equipment: Illumina MiSeqDX (for diagnostic testing)

Pyrosequencing applications

• Quantitative analysis of DNA methylation levels
• Determination of DNA variant allele frequencies (SNPs/ indels) 

Equipment: Qiagen PyroMark Q48 Autoprep System

DNA isolation from a range of sample types (primary cells, cell lines, frozen and paraffin-embedded tissues).

Nucleic acid quantification and quality control assessment.