Affymetrix Microarrays Platform (PAM)

Introducción

  • PAM

PAM is an Affymetrix European Reference Group Cytogenetic applications and participates in the Cytogenetic European Quality Assessment (CEQA)

Affymetrix® Microarrays Platform (PAM) is a service focused on DNA and RNA microarray solutions offered by Affymetrix. Solutions towards a personalized medicine.

Molecular cytogenetics

Microarray studies can offer various solutions for cytogenetic applications:

  • Detection of whole genome gains and losses at a high resolution.
  • Analysis of whole genome absences of heterozygosity.
  • SNPs genotyping and genome-wide association studies.

RNA Analysis Solution

Gene expression profile studies on either human or mouse are suitable for:

  • Detection of genes and pathways involved in diseases, treatment responses and biological processes.
  • Predictive models based on gene expression profiles.
  • Pharmacogenomics and toxicogenomics studies.
  • Alternative splicing detection.
  • Classification of samples on gene signatures.
  • Analysis of miRNA.
  • Microarray analysis on compromised samples with degraded and/or low quantity samples.

Quality sample analysis

In addition, to the microarray procedure we also offer DNA and RNA quantification and quality control analysis.

Contacto

Plataforma Affymetrix de Microarrays (PAM)

IMPPC Building
Ctra. de Can Ruti
Cami de les Escoles s/n
08916 Badalona
Barcelona, Spain
(+34) 93 557 2807 (Mon-Fri 08.30-17.30)

FOR ENQUIRIES AND QUOTES
genomic.platforms@carrerasresearch.org

 
NombreRol
Francesc SoléF_sole_3XCampus Research Director
Mar MalloMmallo_3xPostdoctoral Investigator
Nuri De HaroNuri de HaroCore Facility Technician
Jessica TijeroJessica TijeroCore Facility Technician

Documentos y precios

Servicios

RNA Analysis Solution

RNA Analysis Solution includes whole transcript Expresion Analysis & profiling for both fresh and FFPE samples

- GeneChip® Human/Mouse/Rat Exon 1.0 ST

- GeneChip® Human/Mouse/Rat Gene 1.0 and 2.0 ST

- GeneChip® Human Transcriptome Array 2.0

- Almac Xcel™ Array 

3' IVT Expression Analysis

- GeneChip® PrimeView™ Human Gene Expression Array

- GeneChip® Human Genome U133 Plus 2.0

- miRNA Analysis

- GeneChip® miRNA 3.0 and 4.0 Array

Please see the Affymetrix for other arrays and other species.

Please check the list for a guideline of prices. 

 

Documentación

High throughput qPCR – Biomark HD

-        Genotyping: Genotyping involves the analysis of single nucleotide polymorphisms (SNP) variations in genomes across individual organisms. DNA SNP genotyping on the Biomark HD Module can be performed using assays based on Fluidigm'sSNPtype chemistry or Life Technologie'sTaqMan® technology.

-        Targeted Gene Expression: Real-time quantitative PCR (qPCR) is a powerful technique for quantifying changes in gene expression by producing millions of copies of specific, targeted regions of complementary DNA (cDNA) that has been reverse transcribed from messenger RNA (mRNA) and microRNA (miRNA). Real-time qPCR on the Biomark HD Module can be performed using assays based on Fluidigm's DELTAgene chemistry or Life Technologie'sTaqMan® dual-labeled probes technology.

-        Digital PCR: Digital polymerase chain reaction (dPCR) is a technique that quantifies nucleic acid sequences that are present in a DNA sample. Digital PCR typically relies on standard PCR techniques but increases their sensitivity by dividing a sample into hundreds of smaller reactions and performing a PCR assay on eachsample. Digital PCR applications include copy number variation studies, absolute quantification,mutation detection and study of loss of heterozygosity.