Introduction

The Microarrays Unit (UM) is a service focused on DNA and RNA microarray solutions towards a personalized medicine and participates in the Cytogenetic European Quality Assessment (CEQA).

Molecular cytogenetics

Microarray studies can offer various solutions for cytogenetic applications:

-        Detection of whole genome gains and losses at a high resolution.

-        Analysis of whole genome absences of heterozygosity.

-        SNPs genotyping and genome-wide association studies.

-        RNA Analysis Solution

-        Gene expression profile studies on either human or mouse are suitable for:

-        Detection of genes and pathways involved in diseases, treatment responses and biological processes.

-        Predictive models based on gene expression profiles.

-        Pharmacogenomics and toxicogenomics studies.

-        Alternative splicing detection.

-        Classification of samples on gene signatures.

-        Analysis of miRNA.

-        Microarray analysis on compromised samples with degraded and/or low quantity samples.

-        Quality sample analysis

In addition, to the microarray procedure we also offer:

-        DNA and RNA quantification and quality control analysis.

-        Optical genome mapping (OGM) (Bionano) to detect structural and copy number alterations at a high resolution.

-        Hand made FISH probes from the CHORI BAC collection.

High throughput qPCR

The Biomark HD system is a high throughput qPCR that runs IFCs in either real-time or end-point read modes, bringing PCR solutions to a range of applications. The 48x48 Dynamic Array combines up to 48 samples and 48 assays, generating 2304 different assays in one single run. The 96x96 Dynamic Array combines up to 96 samples and 96 assays, generating 9216 different assays. In addition, the FLEXsix IFC incorporates six 12x12 partitions that can be organized in any configuration, in up to six separate experimental runs.

Applications

-        Genotyping

-        Targeted Gene expression

-        Digital PCR

Equipment

-        Affymetrix/Thermofisher Research Platform: GCS3000 with autoloader

-        Agilent Bioanalyzer 2100

-        NanoDrop 2000 Spectrophotometer

-        Saphyr (Bionano) equipment for OGM. Renting

Petition procedure 

A service request follows the following steps (link)

1. Contact us for an interview with the PI and other participants in the study. Please see the Contact section.

2. Service request sending an e-mail to microarraysunit@carrerasresearch.org and filling in the Service request form.

3. Quotation according the specific needs and type of users.

4. Type of users:

• Can Ruti Campus: Researchers from institutions of the IMPPC immediate surroundings (Institut de Investigació Germans Trias i Pujol and Hospital Germans Trias i Pujol; Institut de Investigació Josep Carreras, etc), or that belong to executive board institutions of the IMPPC Foundation (Universitat Autònoma de Barcelona, etc), as well as researchers who are involved in direct collaborations with IMPPC members and that pay through a shared source of funding.

• External Researchers from all other external public and private institutions.

5. Once the quotation is accepted by the PI, samples will be delivered to the PAM filling in the Sample delivery form and following the samples delivery requirements. Sample extraction is done by the researcher.

6. The sample quality control is required to check that the input sample fulfill all the requirements.

7. Microarray procedure and sample hybridization.

8. Data analysis.

9. Delivery of the report by e-mail or in an interview discussing the results.