Unitat de Proteòmica

Introducció

  • Proteòmica Team

The Proteomics unit of the Josep Carreras Leukemia Research Institute has been created to support the scientific community offering mass spectrometry services to academic and private sector.

In addition, our unit is part of the technological platforms that belong to the Carlos III Health Institute and the Proteomics Network platform (ProteoRed).

One of the main objectives of the unit is to offer a high-quality service in the following disciplines: descriptive proteomics, quantitative proteomics (SILAC, TMT, Label Free), biomarker identification across major human diseases, protein characterization and identification of post-translational modifications (phospho and phospho-enrichment, Acetyl and Acetyl-enrichment, ADP-ribosylation), identification and quantification of protein-protein interactions and identification and further biological validation of bioactive peptides with potential applications in human health care including food supplements and cosmetics, among others.

The proteomics unit, in an effort to implement the Josep Carreras Leukaemia Research Institute philosophy, is working to promote the incorporation of proteomics as a necessary tool for the development of clinical and basic projects in our center. In this line, the implementation of state-of-the-art services allow both clinical and basic researchers to apply these tools to personalized medicine.

Contacte

Unitat de Proteòmica

Edifici IJC
Ctra. de Can Ruti
Cami de les Escoles s/n
08916 Badalona
Barcelona, Spain
(+34) 93 557 2807 (Mon-Fri 08.30-17.30)

PER RESERVES I CONSULTES: cdelatorre@carrerasresearch.org

 

 

NomRol
Carolina De La TorreCarolina de la TorreCore Facility Leader
Joan Josep BechJoan Josep BechPostdoctoral Investigator
Bernat CucurullLogo greenTechnician

Documents i preus

For detailed information about our fees, please fill out the IJC Proteomics Unit Service Request Form (before sending any samples) and send it to:

proteomicsunit@carrerasresearch.org.

Serveis

LC-MSMS proteomics Analyses

1. Protein identification from a single band


2. Protein identification and quantification of complex samples

- iTRAQ-based Proteomics Analysis
- TMT Based Proteomics Analysis
- SILAC-based Proteomics Analysis
- Label-free Quantification
- Parallel Reaction Monitoring (PRM)
- SRM & MRM 

3. Protein Post-translational Modification Analysis:
 
- iTRAQ-based Proteomics Analysis
- TMT Based Proteomics Analysis
- SILAC-based Proteomics Analysis
- Label-free Quantification
- Parallel Reaction Monitoring (PRM)
- SRM & MRM

4. Protein-Protein Interaction Networks: 
 
- Co-immunoprecipitation (co-IP)
- Crosslinking Protein Interaction Analysis
- Pull-Down Assay

5. Protein Post-translational Modification Analysis:
 
- Phosphorylation (phosphoenrichment & phosphorylation site identification)
- Acetylation (Acetyl-Lysine antibody enrichment)
- Methylation
- ADP-ribosylation

Bioinformatics Service for proteomics

1.     Functional annotation and enrichment analysis

2.     Clustering analysis

3.     Network analysis

4.     Statistical analysis

5.     Proteomic analysis of Post-translational Modifications

Proteomics Applications

Biomarkers identification and validation in different kinds of samples:

1. Cells.

2. Fluids (saliva, serum, plasma, urine, interstitial fluids, etc.)

3. Tissues (muscle, brain, lung, kidney, heart, retina, etc.)

4. Fecal.

5. Exosomes.

6. Secretome.

7. Matrisome.