Unidad de Proteómica

Introducción

  • Proteómica Team

Josep Carreras Leukaemia Research Institute (IJC) is working to become a reference institute to apply the precision medicine to leukaemia using different OMICs approaches. The aim of IJC is to promote a competitive proteomics facility that gives support for translational research.

Proteomics techniques have been successfully applied in translational medicine studies and help move towards personalized medicine. Our proteomics unit is involved in various biomedical research areas, giving both scientific and technological support to internal and external researchers and clinicians.

In order to have access to state-of-the-art technology we have a collaboration agreement with some public institutions that belong to the Proteomics Spanish Society (ProteoRed – ISCIII).

Our Advantages

IJC Proteomics unit works closely with you in an integral way from the beginning, in the discussion of the project (including the writing of the project as well as the experimental design), until the last steps providing a detailed report of the final results and the manuscript preparation.

Our Technical staff has extensive experience and we apply the advanced techniques to deliver reliable and high-quality data with cost-efficiency. In addition, we are continuously working to optimize the standard operation procedures and implementing the last methodologies.

In summary, our services include: consultation, sample preparation and mass spectrometry measurement, as well as a standard bioinformatics analysis. Further analyses can be performed in collaboration with the unit.

We look forward to collaborating with you!

Contacto

Unidad de Proteómica

Edificio IJC
Ctra. de Can Ruti
Cami de les Escoles s/n
08916 Badalona
Barcelona, Spain
(+34) 93 557 2807 (Mon-Fri 08.30-17.30)

PARA RESERVAS Y CONSULTAS

cdelatorre@carrerasresearch.org

NombreRol
Carolina De La TorreCarolina de la TorreCore Facility Leader
Joan Josep BechJoan Josep BechPostdoctoral Investigator
Bernat CucurullLogo greenTechnician

Documentos y precios

Servicios

LC-MSMS proteomics Analyses

  1.      Protein identification from a single band

 

  1.      Protein identification and quantification of complex samples:

2.1             iTRAQ-based Proteomics Analysis

2.2             TMT Based Proteomics Analysis

2.3             SILAC-based Proteomics Analysis

2.4             Label-free Quantification

2.5             Parallel Reaction Monitoring (PRM)

2.6             SRM & MRM

 

  1.      Protein-Protein Interaction Networks: 

3.1                    Co-immunoprecipitation (co-IP)

3.2                    Crosslinking Protein Interaction Analysis

3.3                    Pull-Down Assay

  1.      Protein Post-translational Modification Analysis:

 

4.1              Phosphorylation (phosphoenrichment & phosphorylation site identification)

4.2   Acetylation (Acetyl-Lysine antibody enrichment)

4.3    Methylation

4.4   ADP-ribosylation

Bioinformatics Service for proteomics

1.     Functional annotation and enrichment analysis

2.     Clustering analysis

3.     Network analysis

4.     Statistical analysis

5.     Proteomic analysis of Post-translational Modifications

Proteomics Applications

Biomarkers identification and validation in different kinds of samples:

1. Cells.

2. Fluids (saliva, serum, plasma, urine, interstitial fluids, etc.)

3. Tissues (muscle, brain, lung, kidney, heart, retina, etc.)

4. Fecal.

5. Exosomes.

6. Secretome.

7. Matrisome.