Postdoctoral Position in Experimental Biology: 3D Chromatin Organization – Role in Haematological Cancer

Referencia

2019/116

Número de puestos

2

Fecha límite de inscripción

01/11/2019

Requisitos

  • A high level of motivation and interest.
  • PhD in Molecular Biology, Biochemistry, Biotechnology, Biomedicine, Genetics, Genomics or in a related field.
  • Experience in some of these fields: epigenetics (ChIP-seq, RNA-seq, ATAC-seq,…), 3D chromatin organization (Hi-C, UMI-4C, 4C,…), lymphocyte biology (cell isolation, mouse models, FACS,…) or CRISPR technology or shRNAs.
  • Competitive CV with an outstanding publication record
  • High level of collaborative and communicative skills.
  • Good level of English speaking and writing skills.
  • International mobility will be considered a major plus.

Información adicional

Human cells contain about 2 metres of linear DNA not-randomly packed into the nucleus of a few microns diameter, and the manner in which it is wrapped plays a fundamental role in regulating genome expression. In most of the cases, it does this by putting regulatory elements and target gene promoters into physical contact. It has been estimated that the genome harbours around one million regulatory elements, but the vast majority of interactions between these elements and the corresponding regulated gene are uncharted, constituting a major missing link in understanding genome control. The recent development of Promoter Capture Hi-C (PCHi-C) method has allowed for the first time the genome-wide systematic identification of the interacting regions that are in physical contact with 31,253 human promoters (Javierre, Cell 2016). Promoter interactions are highly cell type specific and interacting regions are enriched in noncoding SNPs. This technique has enabled to connect non-coding disease variants to putative target promoters, prioritizing thousands of disease-candidate genes and implicating disease pathways.

 

Chromatin interactions are crucial for cellular health due to their main role in genome expression regulation and errors in these interactions could give rise to the development of a broad range of diseases including blood cancer. For all these reasons, we want to deeply investigate the promoter interactomes in human and mouse haematopoiesis, considering healthy and blood-cancer individuals, and their functional repercussion. This knowledge will help us to improve our knowledge of the haematological cancer process, providing new opportunities for the development of novel treatment approaches and diagnostic strategies. In our approach, we will combine cutting-edge experimental and computational approaches for analysing spatialtemporal  changes in the 3D chromatin packaging and the epigenetic profiles.

 

What we offer

 

  • Incorporation in the ¨3D chromatin group¨ actively involved in wide range of collaborations with different groups of complementary research topics.
  • Exciting and innovative collaborative research project in one of the most promising research topic.
  • Working in basic and clinical research environment of the Josep Carreras Leukaemia Research Institute.
  • The stimulating scientific, social and cultural environment of the Barcelona
  • metropolitan area.
  • Contract details will be negotiated and take into account the track record of the
  • Flexible start date

 

Presentación de las candidaturas

For more information:

 

thttp://www.carrerasresearch.org/es

 

How to apply:

 

Interested applicants should send their CV (containing the contact details of two referees) and a motivation letter: rrhh@carrerasresearch.org

 

PLEASE PUT THE REFERENCE in the subject of the mail or your application may not reach the right person.

 

 

Deadline for applications:

01, november 2019